Cell culture and retroviral transduction of Hut78 cellsHut78 cells were cultured in Rosewell Park Memorial Institute 1640 medium plus 10% FCS and 104 M 2mercaptoethanol (Sigma, Saint Louis, MO, USA). The cells were resuspended at 1 105 cells/ml in the viruscontaining medium diluted 1:2 in DMEM containing 15% FCS and protamine sulfate (5 g/ml) for 4 h. The cells were then incubated at 37 for a further 48 h and fluoresceinactivated cell sorter (FACS) sorting was performed to purify green fluorescent protein (GFP)+ cells from transduced cells.SCHRAGER ON THE SCENE: Mr. Suh! The Radio City crowd reacts with a "Suuuuuuuuuuuuuuuuuuh". I like that.

David Akers misses an early field goal attempt after one drive stalls. He hits his second. Three points early.But there's going to be a number of guys who probably aren't prepared as he might be." . Had the league not granted Fitzgerald early eligibility, he would not have joined Clarett in the courtroom. "I would'v